Comparative assessment of the effect of titanium dioxide – based nanoparticles on boar germ cells in vitro

Modern titanium dioxide nanomaterials, in particular modified with nanosilver, have antiviral, antibacterial, antifungal activity, cytotoxic effects vitro experiments and can affect germ cells of warm-blooded laboratory animals. The research aims to study the effect different types nanoparticles on physiological biochemical characteristics wild boar vitro. When applying maximum dose (LD50) studied nanopowders all samples, sperm survival was probably lower than control: by 41.9% (р<0.001) ‒ under action TiO2 (hereinafter NPs) (Frantsevich Institute for Problems Materials Science NAS Ukraine, hereinafter IPM), 28.0% (р<0.05) NPs (Acros Organics), 53.5% 4% Ag-TiO2 (IPM) 55.9% are 8% (IPM). Nanopowders based manufacturers, characterized similar morphological varying degrees nanosilver addition (Ag content from 0 8%), starting a 1/10 LD50, damaging boars, which is manifested decrease respiratory activity cytochrome oxidase increasing succinate dehydrogenase activity. most sensitive marker rate, value significantly decreased at 1/100 consequence disruption mitochondrial enzymes and, accordingly, resynthesis ATP. Our results confirm extend data nature dioxide-based animals due oxidative stress. obtained experimental will be taken into account hygienic regulation investigated nanopowders.